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lycopersicon esculentum lectin  (Vector Laboratories)


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    Vector Laboratories lycopersicon esculentum lectin
    Lycopersicon Esculentum Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/DL-1177-1/DyLight+594+labeled+Lycopersicon+Esculentum+(Tomato)+Lectin+(LEL%2C+TL)/pm41955093-131-3-7
    Average 96 stars, based on 334 article reviews
    lycopersicon esculentum lectin - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Search for marker proteins to assess blood-brain barrier development.
    Article Snippet: Subsequently, 30 μm thick sagittal sections of the embedded brains were cut, incubated at 24 °C for 3 h in a blocking solution (3% normal goat serum and 0.3% Triton X-100 in phosphate-buffered saline [PBS]), followed by incubation for 1 h at room temperature in a solution containing Alexa 546 streptavidin (1:500; S11225, Invitrogen, Waltham, MA, USA). .. After rinsing, the sections were stained with lectin (1:200; DL1177; Vector Labs, Newark, CA, USA) and 4′,6-diamidino-2-phenylindole (DAPI; 1:1000; 342-07431, Dojindo, Kumamoto, Japan). .. The stained sections were analyzed using a Nikon A1R confocal microscope system (Nikon, Tokyo, Japan) and quantified using NIS-Element analysis software (Nikon).

    Article Title: Improved 3D image reconstruction via deep-learning-based fusion of light-field microscopy and Fourier light-field microscopy images
    Article Snippet: .. This dataset was acquired via confocal microscopy with the following specifications: individual volume dimensions of 1287 × 1287 × 64 pixels , 362 samples in total, and a volume size of 112 × 112 × 57.6 μ m . The imaging was performed using a 40 × / 1.3 NA oil immersion objective, and stained with tomato lectin (DyLight594 conjugated, DL-1177, Vector Laboratories, Newark, California, United States). ..

    Confocal Microscopy:

    Article Title: Improved 3D image reconstruction via deep-learning-based fusion of light-field microscopy and Fourier light-field microscopy images
    Article Snippet: .. This dataset was acquired via confocal microscopy with the following specifications: individual volume dimensions of 1287 × 1287 × 64 pixels , 362 samples in total, and a volume size of 112 × 112 × 57.6 μ m . The imaging was performed using a 40 × / 1.3 NA oil immersion objective, and stained with tomato lectin (DyLight594 conjugated, DL-1177, Vector Laboratories, Newark, California, United States). ..

    Imaging:

    Article Title: Improved 3D image reconstruction via deep-learning-based fusion of light-field microscopy and Fourier light-field microscopy images
    Article Snippet: .. This dataset was acquired via confocal microscopy with the following specifications: individual volume dimensions of 1287 × 1287 × 64 pixels , 362 samples in total, and a volume size of 112 × 112 × 57.6 μ m . The imaging was performed using a 40 × / 1.3 NA oil immersion objective, and stained with tomato lectin (DyLight594 conjugated, DL-1177, Vector Laboratories, Newark, California, United States). ..

    Labeling:

    Article Title: Geometrically Constrained Growth Factor Concentration Favors Enrichment of Goblet Cells and Mucus Formation.
    Article Snippet: Other primary antibodies staining AGR2 (Invitrogen, PA5-34517, 1:200 dilution), RELM-β (Invitrogen, PA561896, 1:200 dilution), and TFF3 (Invitrogen, PA5-57279, 1:200 dilution) were detected by the secondary antibody goat antirabbit IgG conjugated to Alexa Fluor 594 (Jackson ImmunoResearch, 111-585- 003) diluted to 1 μg/mL in 1% BSA in PBS with 1 μg/mL Hoechst 33342 and incubated with the cells for 1 h at 22 °C. .. Fluorescently labeled lectins, Lycopersicon esculentum lectin (LEL, Vector Lab, DL1177, 1:100 dilution), Lotus tetragonolobus lectin (LTL, Vector Lab, FL-1321, 1:200 dilution), and wheat germ agglutinin (WGA, Invitrogen, W11261, 1:100 dilution) lectin were used to label carbohydrate groups on mucins. .. A labeled primary antibody against mucin 1 (Muc1, Invitrogen, sc-7313 AF594, 1:100 dilution) was diluted in IF with 1 μg/mL Hoechst 33342 and incubated with the cells for 24 h at 4 °C.

    Whole Genome Amplification:

    Article Title: Geometrically Constrained Growth Factor Concentration Favors Enrichment of Goblet Cells and Mucus Formation.
    Article Snippet: Other primary antibodies staining AGR2 (Invitrogen, PA5-34517, 1:200 dilution), RELM-β (Invitrogen, PA561896, 1:200 dilution), and TFF3 (Invitrogen, PA5-57279, 1:200 dilution) were detected by the secondary antibody goat antirabbit IgG conjugated to Alexa Fluor 594 (Jackson ImmunoResearch, 111-585- 003) diluted to 1 μg/mL in 1% BSA in PBS with 1 μg/mL Hoechst 33342 and incubated with the cells for 1 h at 22 °C. .. Fluorescently labeled lectins, Lycopersicon esculentum lectin (LEL, Vector Lab, DL1177, 1:100 dilution), Lotus tetragonolobus lectin (LTL, Vector Lab, FL-1321, 1:200 dilution), and wheat germ agglutinin (WGA, Invitrogen, W11261, 1:100 dilution) lectin were used to label carbohydrate groups on mucins. .. A labeled primary antibody against mucin 1 (Muc1, Invitrogen, sc-7313 AF594, 1:100 dilution) was diluted in IF with 1 μg/mL Hoechst 33342 and incubated with the cells for 24 h at 4 °C.

    Incubation:

    Article Title: Search for marker proteins to assess blood-brain barrier development.
    Article Snippet: .. Thereafter, the sections were rinsed and incubated for 1 h at room temperature in a solution containing lectin (1:200; DL1177; Vector Labs) and DAPI (1:1000; 342–07431; Dojindo). .. After a final round of rinsing, drying, and embedding in VectaShield (H-1000; Vector Labs), the stained sections were analyzed using a Nikon A1R-A1 confocal microscope.



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